Proteome network analysis of skeletal muscle in lignan-enriched nutmeg extract-fed aged mice

Abstract Sarcopenia, characterized by reduced muscle mass and fiber number leading to muscular atrophy, has been associated with serious socioeconomic challenges among the elderly in developed countries. Therefore, preventing sarcopenia could be a promising strategy for achieving healthy aging society. Nutmeg ( Myristica fragrans ) used as spice increase flavor prevent putrefaction of food. contains various bioactive components that improve activity. To determine potential effect lignan-enriched nutmeg extract (LNX) on sarcopenia, LNX (100 mg/kg body weight)-fed aged mice were subjected forced exercise. Herein, (22-month-old) fed three weeks exhibited shortened thickened soleus muscle. The ratio (%) weight was significantly increased LNX-fed mice. relative improved exercise activities, including rotarod, swimming, grip strength test results. Proteome profiles analyze protein–protein interaction network. Several myosin heavy chain isoforms found interact actin, ACTA1, which functions hub protein. Furthermore, expression myogenic proteins, such MYH1, MYH4, dose-dependently vivo. In result, our functional proteomic analysis revealed feeding restored proteins